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03294nam a22003375i 4500 |
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978-1-59745-466-7 |
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20191026202041.0 |
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cr nn 008mamaa |
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100301s2007 xxu| s |||| 0|eng d |
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|a 9781597454667
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024 |
7 |
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|a 10.1007/978-1-59745-466-7
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Protein Targeting Protocols
|c edited by Mark van der Giezen.
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250 |
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|a 2nd ed. 2007.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
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|a XV, 501 p.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 390
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|a Bacterial Systems -- Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching -- Membrane Protein Insertion and Secretion in Bacteria -- Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria -- Periplasmic Targeting of Recombinant Proteins in Escherichia coli -- In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export -- Eukaryotic Systems -- Nuclear Import Properties of the Sex-Determining Factor SRY -- The Mitochondrial Machinery for Import of Precursor Proteins -- A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells -- In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria -- Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa -- A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70 -- The Chloroplast Protein Import Machinery: A Review -- Import of Plastid Precursor Proteins Into Pea Chloroplasts -- Protein Targeting in ''Secondary'' or ''Complex'' Chloroplasts -- Protein Trafficking to the Complex Chloroplasts of Euglena -- Fluorescent Protein Fusions for Protein Localization in Plants -- Genetic Transformation -- Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening -- Trafficking Through the Early Secretory Pathway of Mammalian Cells -- Studying Protein Export From the Endoplasmic Reticulum in Plants -- Imaging the Golgi Apparatus in Living Mitotic Cells -- Evaluating Yeast Biosynthetic Vacuolar Transport -- Targeting to Lysosomes in Mammalian Cells -- Monitoring Autophagy in Yeast -- Protein Targeting to Yeast Peroxisomes -- Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins -- General Methods -- Intracellular Protein Localization by Immunoelectron Microscopy -- Separation of Proteins by Blue Native Electrophoresis -- Computational Prediction of Subcellular Localization -- Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes.
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650 |
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|a Proteins .
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650 |
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|a Cell biology.
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650 |
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|a Human genetics.
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650 |
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4 |
|a Protein Science.
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650 |
2 |
4 |
|a Cell Biology.
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650 |
2 |
4 |
|a Human Genetics.
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700 |
1 |
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|a van der Giezen, Mark.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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856 |
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|u https://doi.org/10.1007/978-1-59745-466-7
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