Protein Targeting Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edition: | 2nd ed. 2007. |
| Series: | Methods in Molecular Biology,
390 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-466-7 |
Table of Contents:
- Bacterial Systems
- Localization and Mobility of Bacterial Proteins by Confocal Microscopy and Fluorescence Recovery After Photobleaching
- Membrane Protein Insertion and Secretion in Bacteria
- Identification of Autotransporter Proteins Secreted by Type V Secretion Systems in Gram-Negative Bacteria
- Periplasmic Targeting of Recombinant Proteins in Escherichia coli
- In Vitro Analysis of the Bacterial Twin-Arginine-Dependent Protein Export
- Eukaryotic Systems
- Nuclear Import Properties of the Sex-Determining Factor SRY
- The Mitochondrial Machinery for Import of Precursor Proteins
- A Genetic Method to Identify Mitochondrial Proteins in Living Mammalian Cells
- In Vitro and In Vivo Methods to Study Protein Import Into Plant Mitochondria
- Protein Targeting to Mitochondria of Saccharomyces cerevisiae and Neurospora crassa
- A Mitosome Purification Protocol Based on Percoll Density Gradients and Its Use in Validating the Mitosomal Nature of Entamoeba histolytica Mitochondrial Hsp70
- The Chloroplast Protein Import Machinery: A Review
- Import of Plastid Precursor Proteins Into Pea Chloroplasts
- Protein Targeting in ''Secondary'' or ''Complex'' Chloroplasts
- Protein Trafficking to the Complex Chloroplasts of Euglena
- Fluorescent Protein Fusions for Protein Localization in Plants
- Genetic Transformation
- Identification of Proteins Targeted Into the Endoplasmic Reticulum by cDNA Library Screening
- Trafficking Through the Early Secretory Pathway of Mammalian Cells
- Studying Protein Export From the Endoplasmic Reticulum in Plants
- Imaging the Golgi Apparatus in Living Mitotic Cells
- Evaluating Yeast Biosynthetic Vacuolar Transport
- Targeting to Lysosomes in Mammalian Cells
- Monitoring Autophagy in Yeast
- Protein Targeting to Yeast Peroxisomes
- Expression, Localization, and Topology of Fluorescently Tagged Plasma Membrane-Targeted Transmembrane Proteins
- General Methods
- Intracellular Protein Localization by Immunoelectron Microscopy
- Separation of Proteins by Blue Native Electrophoresis
- Computational Prediction of Subcellular Localization
- Phylogenetic Analysis to Uncover Organellar Origins of Nuclear-Encoded Genes.