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02228nam a22003735i 4500 |
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978-1-59745-494-0 |
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20191028041441.0 |
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cr nn 008mamaa |
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110414s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597454940
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7 |
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|a 10.1007/978-1-59745-494-0
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Transgenic Maize
|b Methods and Protocols /
|c edited by M. Paul Scott.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a IX, 188 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 526
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| 505 |
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|a Design of Gene Constructs for Transgenic Maize -- Transformation Methods -- Transient Expression of GFP in Immature Seed Tissues -- Biolistic Gun-Mediated Maize Genetic Transformation -- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus -- Whiskers-Mediated Maize Transformation -- Transgenic Maize in Research -- Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines -- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression -- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites -- Analysis of Transgenic Plants -- Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize -- Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds -- Determination of Transgene Copy Number by Real-Time Quantitative PCR -- Breeding with Transgenes -- Herbicide Resistance Screening Assay -- Characterizing Transgene Inheritance -- Backcross Breeding.
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| 650 |
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|a Agriculture.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
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|a Plant genetics.
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| 650 |
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|a Transgenic organisms.
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| 650 |
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4 |
|a Agriculture.
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| 650 |
2 |
4 |
|a Plant Sciences.
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| 650 |
2 |
4 |
|a Plant Genetics and Genomics.
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| 650 |
2 |
4 |
|a Transgenics.
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| 700 |
1 |
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|a Scott, M. Paul.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-59745-494-0
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