Transgenic Maize Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edition: | 1st ed. 2009. |
| Series: | Methods in Molecular Biology,
526 |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59745-494-0 |
Table of Contents:
- Design of Gene Constructs for Transgenic Maize
- Transformation Methods
- Transient Expression of GFP in Immature Seed Tissues
- Biolistic Gun-Mediated Maize Genetic Transformation
- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus
- Whiskers-Mediated Maize Transformation
- Transgenic Maize in Research
- Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines
- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression
- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites
- Analysis of Transgenic Plants
- Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize
- Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
- Determination of Transgene Copy Number by Real-Time Quantitative PCR
- Breeding with Transgenes
- Herbicide Resistance Screening Assay
- Characterizing Transgene Inheritance
- Backcross Breeding.