Transgenesis Techniques Principles and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 3rd ed. 2009. |
| Colección: | Methods in Molecular Biology,
561 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-60327-019-9 |
Tabla de Contenidos:
- Transgenesis in Various Model Systems
- Transgenesis in Drosophila melanogaster
- Transgenesis in Caenorhabditis elegans
- Transgenesis in Zebrafish with the Tol2 Transposon System
- Generation of Transgenic Frogs
- Pronuclear DNA Injection for the Production of Transgenic Rats
- Transgenesis in the Mouse
- Cell-Type-Specific Transgenesis in the Mouse
- Transgene Design and Delivery into the Mouse Genome: Keys to Success
- Overexpression Transgenesis in Mouse: Pronuclear Injection
- Gene-Targeting Vectors
- Gene Trap: Knockout on the Fast Lane
- Culture of Murine Embryonic Stem Cells
- Targeting Embryonic Stem Cells
- Generation of Chimeras by Microinjection
- Generation of Chimeras by Morula Aggregation
- Surgical Techniques for the Generation of Mutant Mice
- Site-Specific Recombinases for Manipulation of the Mouse Genome
- Cre Transgenic Mouse Lines
- Large-Scale Mouse Mutagenesis
- Dedicated Mouse Production and Husbandry
- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains
- Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recovery and Distribution of Conserved Mutant Strains.