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03074nam a22003135i 4500 |
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978-1-60761-232-2 |
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20190619151549.0 |
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cr nn 008mamaa |
| 008 |
100715s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781607612322
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| 024 |
7 |
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|a 10.1007/978-1-60761-232-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Reverse Chemical Genetics
|b Methods and Protocols /
|c edited by Hisashi Koga.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XII, 272 p. 64 illus., 4 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 577
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| 505 |
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|a The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics -- ORFeome Cloning -- Systematic Cloning of an ORFeome Using the Gateway System -- High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins -- Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study -- High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data -- Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology -- Modification of cDNA Resources Utilized for Reverse Chemical Genetics -- High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources -- An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources -- Recombinant Protein Production by a Kaiko–Baculovirus System -- Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins -- The Refinement of Established Methods by Recent Innovations -- Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine -- Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis -- Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos -- Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins -- Potential Applicable Techniques for Reverse Chemical Genetics -- Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases -- Preparation of Highly Sensitive Protein Array Using Reactive Polymer -- High-Throughput SPR Biosensor -- Drug Discovery Through Functional Screening in the Drosophila Heart -- Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity -- Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.
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| 650 |
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|a Human genetics.
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| 650 |
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|a Medical genetics.
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| 650 |
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4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Gene Function.
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| 700 |
1 |
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|a Koga, Hisashi.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-60761-232-2
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