Reverse Chemical Genetics Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Koga, Hisashi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 577
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-60761-232-2
Tabla de Contenidos:
  • The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics
  • ORFeome Cloning
  • Systematic Cloning of an ORFeome Using the Gateway System
  • High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins
  • Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study
  • High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data
  • Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology
  • Modification of cDNA Resources Utilized for Reverse Chemical Genetics
  • High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources
  • An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources
  • Recombinant Protein Production by a Kaiko–Baculovirus System
  • Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins
  • The Refinement of Established Methods by Recent Innovations
  • Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
  • Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis
  • Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos
  • Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins
  • Potential Applicable Techniques for Reverse Chemical Genetics
  • Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases
  • Preparation of Highly Sensitive Protein Array Using Reactive Polymer
  • High-Throughput SPR Biosensor
  • Drug Discovery Through Functional Screening in the Drosophila Heart
  • Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity
  • Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety.