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02092nam a22003375i 4500 |
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978-3-642-48848-1 |
| 005 |
20191026142251.0 |
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cr nn 008mamaa |
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121227s1987 gw | s |||| 0|eng d |
| 020 |
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|a 9783642488481
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| 024 |
7 |
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|a 10.1007/978-3-642-48848-1
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Robinson, David G.
|e author.
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| 245 |
1 |
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|a Methods of Preparation for Electron Microscopy
|b An Introduction for the Biomedical Sciences /
|c by David G. Robinson, Ulrich Ehlers, Rainer Herken, Bernd Herrmann, Frank Mayer, Friedrich-Wilhelm Schürmann.
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| 250 |
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|a 1st ed. 1987.
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| 260 |
# |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1987.
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| 300 |
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|a XVIII, 190 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|a 1 An Introduction to Electron Microscopy (EM) -- 1.1 Imaging Methods in Electron Microscopy -- 1.2 Preparation Procedures in TEM -- 1.3 Imaging Problems -- 1.4 Support Films -- 2 Methods for TEM -- 2.1 Fixation, Dehydration and Embedding -- 2.2 Ultramicrotomy -- 2.3 Macromolecular EM -- 2.4 Immunoelectron Microscopy (I EM) -- 2.5 Autoradiography -- 2.6 Freeze (Fracturing) Etching -- 3 Methods for SEM -- 3.1 Conventional Methods of Preparation -- 3.2 Storage of Specimens -- 3.3 Demonstration of Surfaces via Replicas and Casts -- 3.4 Visualization of Internal Surfaces Through Sectioning and Dry-Fracturing (Dry-Cleaving) -- 3.5 Element Analysis -- 4 Evaluation of Micrographs -- 4.1 Morphometry -- 4.2 Averaging and Image Reconstruction -- Appendix: Buffers in Electron Microscopy.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Ehlers, Ulrich.
|e author.
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| 700 |
1 |
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|a Herken, Rainer.
|e author.
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| 700 |
1 |
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|a Herrmann, Bernd.
|e author.
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| 700 |
1 |
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|a Mayer, Frank.
|e author.
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| 700 |
1 |
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|a Schürmann, Friedrich-Wilhelm.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-3-642-48848-1
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