Methods of Preparation for Electron Microscopy An Introduction for the Biomedical Sciences /

Detalles Bibliográficos
Autores principales:	Robinson, David G. (Autor), Ehlers, Ulrich. (Autor), Herken, Rainer. (Autor), Herrmann, Bernd. (Autor), Mayer, Frank. (Autor), Schürmann, Friedrich-Wilhelm. (Autor)
Autor Corporativo:	SpringerLink (Online service)
Formato:	eBook
Lenguaje:	English
Publicado:	Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1987.
Edición:	1st ed. 1987.
Materias:	Cell biology. Cell Biology.
Acceso en línea:	https://doi.org/10.1007/978-3-642-48848-1

Tabla de Contenidos:

1 An Introduction to Electron Microscopy (EM)
1.1 Imaging Methods in Electron Microscopy
1.2 Preparation Procedures in TEM
1.3 Imaging Problems
1.4 Support Films
2 Methods for TEM
2.1 Fixation, Dehydration and Embedding
2.2 Ultramicrotomy
2.3 Macromolecular EM
2.4 Immunoelectron Microscopy (I EM)
2.5 Autoradiography
2.6 Freeze (Fracturing) Etching
3 Methods for SEM
3.1 Conventional Methods of Preparation
3.2 Storage of Specimens
3.3 Demonstration of Surfaces via Replicas and Casts
3.4 Visualization of Internal Surfaces Through Sectioning and Dry-Fracturing (Dry-Cleaving)
3.5 Element Analysis
4 Evaluation of Micrographs
4.1 Morphometry
4.2 Averaging and Image Reconstruction
Appendix: Buffers in Electron Microscopy.

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