Methods of Preparation for Electron Microscopy An Introduction for the Biomedical Sciences /
| Autores principales: | , , , , , |
|---|---|
| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1987.
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| Edición: | 1st ed. 1987. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-48848-1 |
Tabla de Contenidos:
- 1 An Introduction to Electron Microscopy (EM)
- 1.1 Imaging Methods in Electron Microscopy
- 1.2 Preparation Procedures in TEM
- 1.3 Imaging Problems
- 1.4 Support Films
- 2 Methods for TEM
- 2.1 Fixation, Dehydration and Embedding
- 2.2 Ultramicrotomy
- 2.3 Macromolecular EM
- 2.4 Immunoelectron Microscopy (I EM)
- 2.5 Autoradiography
- 2.6 Freeze (Fracturing) Etching
- 3 Methods for SEM
- 3.1 Conventional Methods of Preparation
- 3.2 Storage of Specimens
- 3.3 Demonstration of Surfaces via Replicas and Casts
- 3.4 Visualization of Internal Surfaces Through Sectioning and Dry-Fracturing (Dry-Cleaving)
- 3.5 Element Analysis
- 4 Evaluation of Micrographs
- 4.1 Morphometry
- 4.2 Averaging and Image Reconstruction
- Appendix: Buffers in Electron Microscopy.