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02955nam a22003855i 4500 |
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978-3-642-57783-3 |
| 005 |
20191027042348.0 |
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cr nn 008mamaa |
| 008 |
121227s1996 gw | s |||| 0|eng d |
| 020 |
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|a 9783642577833
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| 024 |
7 |
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|a 10.1007/978-3-642-57783-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Cell Cycle - Materials and Methods
|c edited by Michele Pagano.
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| 250 |
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|a 1st ed. 1996.
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| 260 |
# |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1996.
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| 300 |
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|a XXX, 287 p. 7 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Springer Lab Manuals
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|a One Techniques for Studying the Mammalian Cell Cycle -- 1 Use of Immunohistochemistry in Cell Cycle Studies -- 2 Immunofluorescence Techniques in Cell Cycle Studies -- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes -- 4 Techniques for Monitoring Cell Cycle Phases -- 5 Centrifugal Elutriation -- 6 Synchronization of Mammalian Cells -- 7 Microinjection and Electroporation of Macromolecules into Live Cells -- 8 Stable and Transient Transfections in Cell Cycle Studies -- 9 Quantification of Premature and Normal Mitosis -- Two Techniques for Studying the Xenopus Cell Cycle -- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies -- Three Techniques to Study the Yeast Cell Cycle -- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe -- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe -- Four Techniques for Studying the Cell Cycle of Any Species -- 13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands -- 14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases -- 15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris -- 16 SV40 Based in Vitro DNA Replication Assay -- 17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins -- 18 Purification of Cell Cycle Kinases -- 19 Interaction-Based Cloning of Novel Proteins -- 20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses -- 21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays -- 22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies -- 23 Protein mapping in Cell Cycle Studies -- 24 From Peptide to Purified Antibody.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Microbiology.
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| 650 |
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|a Cancer research.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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|a Animal physiology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Microbiology.
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| 650 |
2 |
4 |
|a Cancer Research.
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| 650 |
2 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Animal Physiology.
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| 700 |
1 |
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|a Pagano, Michele.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-3-642-57783-3
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