Cell Cycle - Materials and Methods
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1996.
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| Edición: | 1st ed. 1996. |
| Colección: | Springer Lab Manuals
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-57783-3 |
Tabla de Contenidos:
- One Techniques for Studying the Mammalian Cell Cycle
- 1 Use of Immunohistochemistry in Cell Cycle Studies
- 2 Immunofluorescence Techniques in Cell Cycle Studies
- 3 Fluorescence in Situ Hybridization and Human Cell Cycle Genes
- 4 Techniques for Monitoring Cell Cycle Phases
- 5 Centrifugal Elutriation
- 6 Synchronization of Mammalian Cells
- 7 Microinjection and Electroporation of Macromolecules into Live Cells
- 8 Stable and Transient Transfections in Cell Cycle Studies
- 9 Quantification of Premature and Normal Mitosis
- Two Techniques for Studying the Xenopus Cell Cycle
- 10 Preparation of Xenopus Egg Extracts and Their Utilization in Cell Cycle Studies
- Three Techniques to Study the Yeast Cell Cycle
- 11 Cloning and Expression of Cell Cycle Genes in Schizosaccharomyces pombe
- 12 Preparation of Synchronous Cultures of the Fission yeast, Schizosaccharomyces Pombe
- Four Techniques for Studying the Cell Cycle of Any Species
- 13 Identification of Novel Cell Cycle Targets Using Small Molecule Ligands
- 14 Bacterial Expression and in Vitro Assay of Cell Cycle Kinases
- 15 Heterologous Expression and Secretion of Cell Cycle Proteins Using the Yeast Pichia pastoris
- 16 SV40 Based in Vitro DNA Replication Assay
- 17 From Sequence to Function-Structural Motifs of Cell Cycle Proteins
- 18 Purification of Cell Cycle Kinases
- 19 Interaction-Based Cloning of Novel Proteins
- 20 Expression of Cell Cycle Proteins Using Recombinant Baculoviruses
- 21 Cell Cycle-Regulated Transcription Factors: Bandshift Assays
- 22 Immunoprecipitation and Immunoblotting in Cell Cycle Studies
- 23 Protein mapping in Cell Cycle Studies
- 24 From Peptide to Purified Antibody.