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03944nam a22003735i 4500 |
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978-3-642-77306-8 |
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20191027201513.0 |
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cr nn 008mamaa |
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121227s1992 gw | s |||| 0|eng d |
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|a 9783642773068
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7 |
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|a 10.1007/978-3-642-77306-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Shivanna, K.R.
|e author.
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| 245 |
1 |
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|a Pollen Biology
|b A Laboratory Manual /
|c by K.R. Shivanna, N.S. Rangaswamy.
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| 250 |
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|a 1st ed. 1992.
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| 260 |
# |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1992.
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| 300 |
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|a XII, 119 p. 11 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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|a 1 Introduction -- 2 Pollen Collection -- 3 In Vitro Germination Methods -- Controlled Hydration/Prehydration -- Culture Medium -- Fixation of Pollen Cultures -- Scoring of Pollen Cultures -- General Precautions -- Methods -- Exercise 1 Hanging Drop Culture -- Exercise 2 Sitting Drop Culture -- Exercise 3 Suspension Culture -- Exercise 4 Surface Culture -- Exercise 5 Estimation of Pollen Tube Growth by the Alcian Blue Method -- 4 Pollen Germination and Pollen Tube Growth in Vitro -- Exercise 6 Effects of Boron and Calcium on Pollen Germination and Pollen Tube Growth -- Exercise 7 Leaching of Pollen Substances in Vitro -- Exercise 8 Regulation of pH by Pollen Leachate -- Exercise 9 Role of Transcription and Translation in Pollen Germination and Pollen Tube Growth -- 5 Tests for Pollen Viability -- Exercise 10 In Vitro Germination Test -- Exercise 11 Tetrazolium Test -- Exercise 12 Fluorochromatic Reaction (FCR) Test -- Exercise 13 Fruit Set and Seed Set Test -- Exercise 14 In Vivo Germination and Pollen Tube Growth -- 6 Pollen Storage -- Exercise 15 Effects of Temperature and RH on Viability of Stored Pollen -- Exercise 16 Storage of Pollen Grains in Organic Solvents -- 7 Pollen-Pistil Interaction -- Exercise 17 Cytochemical Localization of Esterases on Stigma Surface -- Exercise 18 Aniline Blue Fluorescence Method to Study Pollen Germination and Pollen Tube Growth in the Pistil -- Exercise 19 Multiple Staining for Localizing Pollen Tubes in the Pistil -- Exercise 20 Semi-Vivo Technique to Study Pollen Germination and Pollen Tube Growth -- Exercise 21 Use of DNA Fluorochromes for Studies on Pollen Grain and Pollen Tube Nuclei -- Exercise 22 Controlled Pollinations -- 8 Self-Incompatibility -- Exercise 23 Confirmation of Self-Incompatibility -- Exercise 24 Studies on Heteromorphic Self-Incompatibility -- Exercise 25 Bud Pollination and Delayed Pollination to Overcome Self-Incompatibility -- Exercise 26 In Vitro Placental Pollination and Fertilization -- 9 Isolation of Protoplasts -- Exercise 27 Isolation of Microspore Protoplasts -- Exercise 28 Isolation of Protoplasts from Pollen Grains -- Exercise 29 Isolation of Sperm Cells (Male Gametes) -- Exercise 30 Isolation of Embryo Sacs and Protoplasts from Embryo Sacs -- 10 Appendices -- Appendix 1 Basic Requirements for Laboratory Exercises -- Appendix 2 Suitable Plant Systems -- Appendix 3 Pollen Morphology -- Appendix 4 Pollen Counts -- Appendix 5 Methods to Obtain Constant Relative Humidity -- Appendix 6 Principles of Fluorescence Microscopy -- Appendix 7 Commonly Used Fluorochromes, and Their Excitation and Fluorescence Ranges -- Appendix 8 Filter Combinations Commonly Used for Different Excitation Ranges -- Appendix 9 Basic Statistical Analyses -- References.
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| 650 |
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0 |
|a Agriculture.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
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|a Zoology.
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| 650 |
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0 |
|a Forestry.
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| 650 |
1 |
4 |
|a Agriculture.
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| 650 |
2 |
4 |
|a Plant Sciences.
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| 650 |
2 |
4 |
|a Zoology.
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| 650 |
2 |
4 |
|a Forestry.
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| 700 |
1 |
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|a Rangaswamy, N.S.
|e author.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-3-642-77306-8
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