Pollen Biology A Laboratory Manual /
| Autores principales: | , |
|---|---|
| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1992.
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| Edición: | 1st ed. 1992. |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-77306-8 |
Tabla de Contenidos:
- 1 Introduction
- 2 Pollen Collection
- 3 In Vitro Germination Methods
- Controlled Hydration/Prehydration
- Culture Medium
- Fixation of Pollen Cultures
- Scoring of Pollen Cultures
- General Precautions
- Methods
- Exercise 1 Hanging Drop Culture
- Exercise 2 Sitting Drop Culture
- Exercise 3 Suspension Culture
- Exercise 4 Surface Culture
- Exercise 5 Estimation of Pollen Tube Growth by the Alcian Blue Method
- 4 Pollen Germination and Pollen Tube Growth in Vitro
- Exercise 6 Effects of Boron and Calcium on Pollen Germination and Pollen Tube Growth
- Exercise 7 Leaching of Pollen Substances in Vitro
- Exercise 8 Regulation of pH by Pollen Leachate
- Exercise 9 Role of Transcription and Translation in Pollen Germination and Pollen Tube Growth
- 5 Tests for Pollen Viability
- Exercise 10 In Vitro Germination Test
- Exercise 11 Tetrazolium Test
- Exercise 12 Fluorochromatic Reaction (FCR) Test
- Exercise 13 Fruit Set and Seed Set Test
- Exercise 14 In Vivo Germination and Pollen Tube Growth
- 6 Pollen Storage
- Exercise 15 Effects of Temperature and RH on Viability of Stored Pollen
- Exercise 16 Storage of Pollen Grains in Organic Solvents
- 7 Pollen-Pistil Interaction
- Exercise 17 Cytochemical Localization of Esterases on Stigma Surface
- Exercise 18 Aniline Blue Fluorescence Method to Study Pollen Germination and Pollen Tube Growth in the Pistil
- Exercise 19 Multiple Staining for Localizing Pollen Tubes in the Pistil
- Exercise 20 Semi-Vivo Technique to Study Pollen Germination and Pollen Tube Growth
- Exercise 21 Use of DNA Fluorochromes for Studies on Pollen Grain and Pollen Tube Nuclei
- Exercise 22 Controlled Pollinations
- 8 Self-Incompatibility
- Exercise 23 Confirmation of Self-Incompatibility
- Exercise 24 Studies on Heteromorphic Self-Incompatibility
- Exercise 25 Bud Pollination and Delayed Pollination to Overcome Self-Incompatibility
- Exercise 26 In Vitro Placental Pollination and Fertilization
- 9 Isolation of Protoplasts
- Exercise 27 Isolation of Microspore Protoplasts
- Exercise 28 Isolation of Protoplasts from Pollen Grains
- Exercise 29 Isolation of Sperm Cells (Male Gametes)
- Exercise 30 Isolation of Embryo Sacs and Protoplasts from Embryo Sacs
- 10 Appendices
- Appendix 1 Basic Requirements for Laboratory Exercises
- Appendix 2 Suitable Plant Systems
- Appendix 3 Pollen Morphology
- Appendix 4 Pollen Counts
- Appendix 5 Methods to Obtain Constant Relative Humidity
- Appendix 6 Principles of Fluorescence Microscopy
- Appendix 7 Commonly Used Fluorochromes, and Their Excitation and Fluorescence Ranges
- Appendix 8 Filter Combinations Commonly Used for Different Excitation Ranges
- Appendix 9 Basic Statistical Analyses
- References.