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03841nam a22003015i 4500 |
| 001 |
978-3-642-80343-7 |
| 005 |
20191028021343.0 |
| 007 |
cr nn 008mamaa |
| 008 |
121227s1998 gw | s |||| 0|eng d |
| 020 |
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|a 9783642803437
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| 024 |
7 |
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|a 10.1007/978-3-642-80343-7
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Microinjection and Transgenesis
|b Strategies and Protocols /
|c edited by Angel Cid-Arregui, Alejandro Garcia-Carranca.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
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|a Berlin, Heidelberg :
|b Springer Berlin Heidelberg :
|b Imprint: Springer,
|c 1998.
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| 300 |
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|a XIV, 619 p. 66 illus., 10 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Springer Lab Manuals
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| 505 |
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|a 1 Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture -- 1 Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells -- 2 Automated Computer-Assisted Microinjection into Cultured Somatic Cells -- 3 Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells -- 4 Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture -- 5 Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments -- 6 Colorimetric in Situ Hybridization -- 7 Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells -- 8 Cellular Microbiochemistry -- 9 A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth -- 10 The Use of Microinjection to Study Signal Transduction in Mammalian Cells -- 2 Strategies and Protocols for Microinjection Experiments Using Embryos -- 11 Supply and Husbandry of Mice for Transgenic Science -- 12 Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis? -- 13 Sperm-Mediated Gene Transfer -- 14 Application of Ligand-Dependent Site-Specific Recombination in ES Cells -- 15 A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos -- 16 The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells -- 17 The Generation of Transgenic Mice with Yeast Artificial Chromosomes -- 18 Following Up Microinjected mRNAs: Analysis of mRNA Poly(A) Tail Length During Mouse Oocyte Maturation -- 19 Mouse Preimplantation Embryos and Oocytes as an In Vivo System to Study Transcriptional Enhancers -- 20 The Use of Mouse Preimplantation Embryos for the Identification of DNA-Binding Proteins Essential in Early Development -- 21 Application of Transgenes and Transgenic Mice to Study Gene Activity from the Oocyte to the Early Embryo -- 22 Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements -- 23 Transgenic Analysis of Embryonic Gene Expression Using LacZ as a Reporter -- 24 Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development -- 25 Multiple Developmental Functions of the Zinc Finger Gene Krox-20 Are Revealed by Targeted Insertion of the E. coli lacZ Coding Sequence -- 26 Transgenic Mouse Strategies in Virus Research -- 27 Isolation of Mutated Genes from Transgene Insertion Sites -- 28 Contributions of Transgenic and Knockout Mice to Immunological Knowledge -- 29 Generation and Application of Transgenic Rabbits -- 30 Microinjection of Drosophila Eggs.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Cid-Arregui, Angel.
|e editor.
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| 700 |
1 |
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|a Garcia-Carranca, Alejandro.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-3-642-80343-7
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