Microinjection and Transgenesis Strategies and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Berlin, Heidelberg :
Springer Berlin Heidelberg : Imprint: Springer,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Springer Lab Manuals
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| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-3-642-80343-7 |
Tabla de Contenidos:
- 1 Strategies and Protocols for Microinjection Experiments Using Somatic Cells in Culture
- 1 Protocols for the Manual and Automatic Microinjection of Somatic Cells in Culture and for the Analysis of Microinjected Cells
- 2 Automated Computer-Assisted Microinjection into Cultured Somatic Cells
- 3 Immunofluorescence and Immunoelectron Microscopy of Microinjected and Transfected Cultured Cells
- 4 Videomicroscopy of Living Microinjected Hippocampal Neurons in Culture
- 5 Preparation, Purification, and Analysis of Synthetic Oligonucleotides Suitable for Microinjection Experiments
- 6 Colorimetric in Situ Hybridization
- 7 Manual Microinjection: Measuring the Inhibitory Effect of HIV-1-Directed Hammerhead Ribozymes in Tissue Culture Cells
- 8 Cellular Microbiochemistry
- 9 A New Strategy for Studying Microtubule Transport and Assembly During Axon Growth
- 10 The Use of Microinjection to Study Signal Transduction in Mammalian Cells
- 2 Strategies and Protocols for Microinjection Experiments Using Embryos
- 11 Supply and Husbandry of Mice for Transgenic Science
- 12 Mouse Male Germ Cells in Culture: Toward a New Approach in Transgenesis?
- 13 Sperm-Mediated Gene Transfer
- 14 Application of Ligand-Dependent Site-Specific Recombination in ES Cells
- 15 A Modified Protocol for the Generation of Transgenic Mice and Freezing of Mouse Embryos
- 16 The Use of Morula Aggregation to Generate Germline Chimeras from Genetically Modified Embryonic Stem Cells
- 17 The Generation of Transgenic Mice with Yeast Artificial Chromosomes
- 18 Following Up Microinjected mRNAs: Analysis of mRNA Poly(A) Tail Length During Mouse Oocyte Maturation
- 19 Mouse Preimplantation Embryos and Oocytes as an In Vivo System to Study Transcriptional Enhancers
- 20 The Use of Mouse Preimplantation Embryos for the Identification of DNA-Binding Proteins Essential in Early Development
- 21 Application of Transgenes and Transgenic Mice to Study Gene Activity from the Oocyte to the Early Embryo
- 22 Transitory Transgenic Analysis as an In Vivo System to Study Promoter Regulatory Elements
- 23 Transgenic Analysis of Embryonic Gene Expression Using LacZ as a Reporter
- 24 Autonomous Cell Labeling Using a laacZ Reporter Transgene to Produce Genetic Mosaics During Development
- 25 Multiple Developmental Functions of the Zinc Finger Gene Krox-20 Are Revealed by Targeted Insertion of the E. coli lacZ Coding Sequence
- 26 Transgenic Mouse Strategies in Virus Research
- 27 Isolation of Mutated Genes from Transgene Insertion Sites
- 28 Contributions of Transgenic and Knockout Mice to Immunological Knowledge
- 29 Generation and Application of Transgenic Rabbits
- 30 Microinjection of Drosophila Eggs.