miRNA Maturation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Arenz, Christoph. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1095
Materias:
Tabla de Contenidos:
  • The Pathway of miRNA Maturation
  • Micro RNA Maturation and Human Disease
  • Approaches to the Modulation of miRNA Maturation
  • Expression Profiling of Components of the miRNA Maturation Machinery
  • Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8
  • In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity
  • Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates
  • A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer
  • Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification
  • Identification of Inhibitors of microRNA Function from Small Molecule Screens
  • Quantitative RT-PCR Specific for Precursor and Mature miRNAs
  • Cellular MicroRNA Sensors Based on Luciferase Reporters
  • Inhibition of miRNA maturation by Peptide Nucleic Acids
  • Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs
  • Lentiviral Overexpression of miRNAs.