Neurotrophin Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Rush, Robert A. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edition:1st ed. 2001.
Series:Methods in Molecular Biology, 169
Subjects:
Online Access:https://doi.org/10.1385/1592590608
Table of Contents:
  • Neurotrophin Quantification and Localization
  • Localization of Neurotrophin Proteins Within the Central Nervous System by Immunohistochemistry
  • Neurotrophin Immunohistochemistry in Peripheral Tissues
  • Extraction and Quantification of the Neurotrophins
  • Identifying Novel Proteins in Nervous Tissue Using Microsequencing Techniques
  • RNA Quantification and Localization
  • Quantification of mRNA Levels Using Northern Blotting
  • Quantification of mRNA Levels Using Ribonuclease Protection Assay
  • Quantification of Neurotrophin mRNA by RT-PCR
  • Sensitive and Nonradioactive In Situ Detection of Neurotrophin mRNAs in the Nervous System
  • Construction of Recombinant Proteins,and Transgenic Cells and Animals
  • Expression of Neurotrophic Factors such as GDNF in the E. coli System
  • Construction of Cells Expressing Neurotrophins
  • Efficient Generation of Stable Pheochromocytoma (PC12) Cell Lines Using a Recombinant Retrovirus (LNC)
  • Construction and Analysis of Transgenic Animals Overexpressing Neurotrophins
  • Construction of Transgenic Animals Overproducing Neurotrophins and Their Receptors
  • In Vivo Analysis
  • Tracing with Radiolabeled Neurotrophins
  • Toxin-Induced Death of Neurotrophin-Sensitive Neurons
  • Oligonucleotides as Inhibitors of Protein Synthesis
  • The IODO-GEN Method for Labeling and the Use of Retrograde Axonal Transport to Assay Neurotrophins
  • Stereological Estimation of Neuronal Number The Optical Disector/Cavalieri Combination.