Methods of Preparation for Electron Microscopy An Introduction for the Biomedical Sciences /

Methods of Preparation for Electron Microscopy An Introduction for the Biomedical Sciences /

Bibliographic Details
Main Authors: Robinson, David G. (Author), Ehlers, Ulrich. (Author), Herken, Rainer. (Author), Herrmann, Bernd. (Author), Mayer, Frank. (Author), Schürmann, Friedrich-Wilhelm. (Author)
Corporate Author: SpringerLink (Online service)
Format: eBook
Language:English
Published: Berlin, Heidelberg : Springer Berlin Heidelberg : Imprint: Springer, 1987.
Edition:1st ed. 1987.
Subjects:
Cell biology.
Cell Biology.
Online Access:https://doi.org/10.1007/978-3-642-48848-1
Table of Contents:
  • 1 An Introduction to Electron Microscopy (EM)
  • 1.1 Imaging Methods in Electron Microscopy
  • 1.2 Preparation Procedures in TEM
  • 1.3 Imaging Problems
  • 1.4 Support Films
  • 2 Methods for TEM
  • 2.1 Fixation, Dehydration and Embedding
  • 2.2 Ultramicrotomy
  • 2.3 Macromolecular EM
  • 2.4 Immunoelectron Microscopy (I EM)
  • 2.5 Autoradiography
  • 2.6 Freeze (Fracturing) Etching
  • 3 Methods for SEM
  • 3.1 Conventional Methods of Preparation
  • 3.2 Storage of Specimens
  • 3.3 Demonstration of Surfaces via Replicas and Casts
  • 3.4 Visualization of Internal Surfaces Through Sectioning and Dry-Fracturing (Dry-Cleaving)
  • 3.5 Element Analysis
  • 4 Evaluation of Micrographs
  • 4.1 Morphometry
  • 4.2 Averaging and Image Reconstruction
  • Appendix: Buffers in Electron Microscopy.

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